Glycerin

The most effective and best-studied humectant in skincare. Glycerin (glycerol) is a small molecule that penetrates the stratum corneum readily, drawing water from the atmosphere and deeper skin layers to hydrate the outer skin. Present in virtually every moisturizer and many serums. Studies show it rivals or outperforms hyaluronic acid for hydration efficacy at a fraction of the cost.

Concentration & Usage

Concentrations: 2-10% in most products (often higher in moisturizers). Pure glycerin is too sticky — always diluted in formulations. Works best below 20% concentration. No maximum daily use limit.

Application

Topical (present in virtually all moisturizers, serums, and toners). Apply as part of regular skincare routine.

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Mechanism of Action

Glycerin (glycerol) is a small three-carbon polyol (92 Da) that penetrates the stratum corneum via intercellular lipid and transcellular routes. It acts as a humectant through osmotic mechanisms—creating a concentration gradient that draws water from the atmosphere (when humidity >65%) and from deeper epidermal layers. Glycerin upregulates aquaporin-3 (AQP3), a glycerol-permeable water channel in keratinocyte membranes that facilitates water and glycerol transport. It supports the natural moisturizing factor (NMF)—amino acids, lactate, and urea that maintain stratum corneum water-holding capacity. Glycerin modulates keratinocyte differentiation and may enhance lipid lamellae organization. Mild antimicrobial activity occurs through osmotic stress. Clinical studies demonstrate hydration efficacy comparable to or exceeding hyaluronic acid.

Ingredient Interactions

Hyaluronic AcidExcellent Combo

Both draw moisture into the skin but through different mechanisms. Glycerin is a smaller molecule and provides consistent hydration; HA holds up to 1000x its weight in water at the surface.

See our full Ingredient Interactions Guide for more combinations and timing strategies.

Regulatory Status

GRAS ingredient. Cosmetic ingredient available worldwide. One of the oldest and most established humectants.

Risks & Safety

Common

Stickiness at high concentrations. May cause mild breakouts in some acne-prone individuals (varies by person).

Serious

None. Extremely safe.

Rare

Allergic reaction is extremely rare.

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Research Papers

9
Safety Assessment of Glycerin as Used in Cosmetics.

Published: December 16, 2019

AI Summary

The Cosmetic Ingredient Review Expert Panel (Panel) reviewed relevant animal and human data. The Panel concluded that glycerin is safe as a cosmetic ingredient in the practices of use and concentration described in this safety assessment.

Maintaining skin integrity in the aged: A systematic review.

Published: March 22, 2020

AI Summary

In aged nursing care receivers, the prevalence of adverse skin conditions such as xerosis cutis, intertrigo, pressure ulcers or skin tears is high. Adequate skin care strategies are an effective method for maintaining and enhancing skin health and integrity in this population.

Treatment of seborrheic dermatitis: a comprehensive review.

Published: March 23, 2019

AI Summary

Seborrheic dermatitis (SD) is a chronic, recurring inflammatory skin disorder that manifests as erythematous macules or plaques with varying levels of scaling associated with pruritus. SD treatment focuses on clearing signs of the disease; ameliorating associated symptoms, such as pruritus; and maintaining remission with long-term therapy.

One Acne™: A holistic management approach to improve overall skin quality and treatment outcomes in acne with or without sensitive skin.

Published: April 16, 2025

AI Summary

Acne and sensitive skin can take a profound toll on patients' well-being, which can be exacerbated if the conditions are experienced together. Moreover, some corrective procedures, e.g., injectable non-animal stabilized hyaluronic acid (NASHA) fillers, have proven effective for treating acne scarring.

Promising Essential Oils/Plant Extracts in the Prevention and Treatment of Dandruff Pathogenesis.

Published: October 28, 2022

AI Summary

Topical agents and synthetic drugs used for dandruff treatment have specific side effects including burning at the application site, depression, dizziness, headache, itching or skin rash, nausea, stomach pain, vision change, vomiting, discoloration of hair, dryness or oiliness of the scalp and increased loss of hair.

Survival and unique clinical practices of extremely preterm infants born at 22-23 weeks' gestation in Japan: a national survey.

Published: December 19, 2024

AI Summary

To investigate prognosis and clinical practices of infants born at 22-23 weeks' gestational age (wkGA) in Japan.

Ozonized glycerin (OG)-based cosmetic products lighten age spots on human facial skin.

Published: July 1, 2022

AI Summary

Few cosmetic ingredients are shown to be able to safely remove or lighten facial dark spots once they have formed. OG has been reported to possess oxidation power and exhibit various biological activities such as antibacterial, antiviral, and wound healing promotion.

Carbonylation of skin collagen induced by reaction with methylglyoxal.

Published: July 11, 2021

AI Summary

Analysis of the structure of AGEs on the carbonylated proteins extracted from MGO-treated skin sections revealed that skin collagen had been converted to MG-H1-modified protein. These novel findings suggest that continuous application of MGO to the skin leads to carbonylation of proteins, which may cause prompt accumulation of MG-H1-modified der...

The Role of Moisturizer Containing Anti-inflammatory on Skin Hydration in Mild-Moderate Atopic Dermatitis Patients.

Published: December 22, 2024

AI Summary

There was also a significant difference in the improvement of skin hydration outcomes between both groups (p < 0.05). The severity of the disease using Scoring Atopic Dermatitis (SCORAD) showed a significant difference (p < 0.05) between the experiment group and the control group after 2 weeks of intervention.

Frequently Asked Questions

What is Glycerin used for?

The most effective and best-studied humectant in skincare. Glycerin (glycerol) is a small molecule that penetrates the stratum corneum readily, drawing water from the atmosphere and deeper skin layers to hydrate the outer skin. Present in virtually every moisturizer and many serums. Studies show it rivals or outperforms hyaluronic acid for hydration efficacy at a fraction of the cost.

What are the side effects of Glycerin?

Common: Stickiness at high concentrations. May cause mild breakouts in some acne-prone individuals (varies by person). Serious: None. Extremely safe. Rare: Allergic reaction is extremely rare.

How is Glycerin administered?

Glycerin is administered via topical (present in virtually all moisturizers, serums, and toners). apply as part of regular skincare routine..

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